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Service ( 服务 )

The laboratory provides the following service:

  • BAC library construction
  • PCR screening of BAC/BIBAC libraries
  • BAC sequencing
  • BAC end sequencing
  • BAC fingerprinting and Physical mapping

You provide primer information and receive BAC/BIBAC clones and sequences.

本实验室提供以下服务:

  • BAC文库构建
  • BAC / BIBAC 文库的PCR筛选
  • BAC测序
  • BAC末端测序
  • BAC指纹图谱制作和物理图谱制作

您提供引物信息即可获得BAC/BIBAC克隆和序列。

Introduction ( 简介 )

The Genome Resource laboratory was started to set up in 2005. Its research focuses on the creation and utilization of genome resources and functional identification and characterization of plant chloroplast proteins.

本基因组资源实验室始建于2005年,其研究集中在基因组资源的创建与应用和叶绿体蛋白的功能鉴定与分析。

Large DNA fragment BAC (Bacterial Artificial Chromosome) and BIBAC (Binary BAC) libraries are important genome resources. They play important roles in whole or targeted genome sequencing, whole large gene cloning and analysis, genome comparative studies, positional cloning of functional genes, genetic resource transfer between organisms of far phylogenetic distances etc. Currently, the laboratory has established the first full public BAC resource platform in China for high-quality BAC/BIBAC library construction, BAC end sequencing, BAC fingerprinting and BAC physical map construction, has created a large amount of BAC/BIBAC resources for important cereal plants, and is using the resources to perform genome comparative studies, clone genes and transfer large DNA fragments between genus. All the resources are available to public.

大片段DNA BAC(细菌人工染色体)和BIBAC(双元BAC)文库是重要的基因组资源,在全基因组和目标区段测序、完整基因克隆分析、基因组比较研究、功能基因原位克隆、远缘基因资源的遗传转移等方面起着重要的作用。目前,本实验室已建立起国内第一个从高质量BAC/BIBAC文库构建到BAC末端测序、BAC指纹制作再到BAC物理图谱制作的完整的公共BAC资源平台,已创建了大量重要禾谷类植物的BAC/BIBAC资源,并在利用这些资源进行基因组比较研究、基因克隆和属间大片段DNA转移等。这些资源也均已对研究人员开放。

The chloroplast is the place for photosynthesis that provides biochemical energy for human/animal living and the development of its host plants. It contains about 3,000 proteins with ~95% encoded by the nuclear genome. The functions of the most chloroplast proteins are not clear. The laboratory is now exploring the functional networks, and physiological and biochemical roles of the chloroplast proteins using a combination of methods of genomics, genetics, molecular biology and biochemistry with Arabidopsis, pea, Chlamydomonas and rice as model systems.

叶绿体是光合作用的场所,光合作用为人类和动物的生存以及植物自身的生长发育提供所必需的生物化学能源。叶绿体含有约3000个蛋白质,这些蛋白质大约有95%由细胞核基因编码,绝大部分叶绿体蛋白质的功能尚不清楚。本实验室正综合利用拟南芥、豌豆、依藻和水稻等各种模式植物结合基因组学、遗传学、分子生物学和生物化学等方法对叶绿体蛋白质的功能网络和生理生化作用进行研究。