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Service ( 服务 )

The laboratory provides the following service:

  • BAC library construction
  • PCR screening of BAC/BIBAC libraries
  • BAC sequencing
  • BAC end sequencing
  • BAC fingerprinting and Physical mapping

You provide primer information and receive BAC/BIBAC clones and sequences.


  • BAC文库构建
  • BAC / BIBAC 文库的PCR筛选
  • BAC测序
  • BAC末端测序
  • BAC指纹图谱制作和物理图谱制作


     E-mail: mzluo@mail.hzau.edu.cn
     Tel: +86 27 87284213(office)
         +86 27 87282936(lab)

Introduction ( 简介 )

The Genome Resource laboratory was started to set up in 2005. Its research focuses on the creation and utilization of genome resources and functional identification and characterization of plant chloroplast proteins.


Large DNA fragment BAC (Bacterial Artificial Chromosome) and BIBAC (Binary BAC) libraries are important genome resources. They play important roles in whole or targeted genome sequencing, whole large gene cloning and analysis, genome comparative studies, positional cloning of functional genes, genetic resource transfer between organisms of far phylogenetic distances etc. Currently, the laboratory has established the first full public BAC resource platform in China for high-quality BAC/BIBAC library construction, BAC end sequencing, BAC fingerprinting and BAC physical map construction, has created a large amount of BAC/BIBAC resources for important cereal plants, and is using the resources to perform genome comparative studies, clone genes and transfer large DNA fragments between genus. All the resources are available to public.

大片段DNA BAC(细菌人工染色体)和BIBAC(双元BAC)文库是重要的基因组资源,在全基因组和目标区段测序、完整基因克隆分析、基因组比较研究、功能基因原位克隆、远缘基因资源的遗传转移等方面起着重要的作用。目前,本实验室已建立起国内第一个从高质量BAC/BIBAC文库构建到BAC末端测序、BAC指纹制作再到BAC物理图谱制作的完整的公共BAC资源平台,已创建了大量重要禾谷类植物的BAC/BIBAC资源,并在利用这些资源进行基因组比较研究、基因克隆和属间大片段DNA转移等。这些资源也均已对研究人员开放。

The chloroplast is the place for photosynthesis that provides biochemical energy for human/animal living and the development of its host plants. It contains about 3,000 proteins with ~95% encoded by the nuclear genome. The functions of the most chloroplast proteins are not clear. The laboratory is now exploring the functional networks, and physiological and biochemical roles of the chloroplast proteins using a combination of methods of genomics, genetics, molecular biology and biochemistry with Arabidopsis, pea, Chlamydomonas and rice as model systems.